Authors
- Klychenkov Sergey Viktorovich
- Kruchinina Anastasia Dmitriyevna PhD of Biological Sciences
- Gamzin Sergey Sergeevich PhD of Biological Sciences
- Levashova Olga Anatolyevna PhD of Biological Sciences
Annotation
Over the past few years, the incidence of anxiety and depressive disorders has increased dramatically, so the study of the anxiolytic activity of peptides of various origins is an urgent problem. The purpose of this article is to study the effect of peptides of royal jelly, drone brood and bee honey weighing up to 5 kDa on the activity of carboxypeptidases E and D in the nervous tissue and adrenal glands of rats under chronic stress. For this purpose, male rats of the Wistar line were subjected to random moderate stress for 16 days and at the same time, once a day, intranasally received aqueous solutions of the listed peptides at a concentration of 300 micrograms / kg of body weight. On day 17, the animals were removed from the experiment and their tissue samples were used to measure the activity of carboxypeptidases and the level of stress hormones in the blood serum. It was found that the enzymatic activity in the pituitary gland, amygdala and medulla oblongata under the action of peptides of drone brood and royal jelly is lower by 61 % / 70 %, 53 % / 44 %, and 37% / 44%, respectively, and in the hypothalamus, striatum, hippocampus and quadriplegia — higher by 94% / 172 %, 86 % / 114 %, 116 % / 189 %, 96 % / 54 % accordingly, compared with the animals of the control group. The effect of bee honey peptides
on enzymatic activity was not established, as well as the effect of all the peptides studied on the activity of carboxypeptidases in the adrenal glands and the level of corticosterone and ACTH in the blood serum. Thus, as a result of this experiment, it was shown that peptides of bee products affect the activity of carboxypeptidases E and D in the nervous tissue of rats, but not in all departments, and do not affect the work of the hypothalamic-pituitary-adrenal axis. The obtained data can be used as an experimental basis for the creation of a biologically active additive with anxiolytic action.
How to link insert
Klychenkov, S. V., Kruchinina, A. D., Gamzin, S. S. & Levashova, O. A. (2023). Evaluation of changes in the activity of carboxypeptidases under the action of peptides of bee products under chronic stress Bulletin of the Moscow City Pedagogical University. Series "Pedagogy and Psychology", 2023, №3 (51), 24. https://doi.org/10.25688/2076-9091.2023.51.3.02
References
1.
1. Klychenkov S. V., Kruchinina A. D. Vliianie peptidov produktov pchelovodstva na stepen’ razvitiia angedonii u krys v usloviiakh khronicheskogo stressa // Scientifical Aspect. 2022. Т. 5. № 5. 601–601. (In Russ.). URL: https://elibrary.ru/item.asp?id=49848131
2.
2. Klychenkov S. V., Kruchinina A. D. Sposob vydeleniia i ochistki nizkomolekuliarnykh peptidov iz produktov pchelovodstva s ispol’zovaniem khromatograficheskikh metodov // Sorbtsionnye i Khromatograficheskie Protsessy, 2023. Т. 23. № 1. S. 107–115. (In Russ.). DOI: /10.17308/sorpchrom.2023.23/10998
3.
3. Kruchinina A. D., Gengin M. T. The effect of a single administration of fluoxetine on the activity of carboxypeptidase E in the nervous tissue of rats // Neurochemical Journal. 2015. Т. 32. № 4. S. 307–311. (In Russ.). DOI: 10.7868/S102781331504010X
4.
4. Bosch E. BDV Syndrome: An Emerging Syndrome with Profound Obesity and Neurodevelopmental Delay Resembling Prader — Willi Syndrome / E. Bosch, M. Hebebrand, B. Popp et al. // Journal of Clinical Endocrinology and Metabolism. 2021. Vol. 106. Issue 12. P. 3413–3427. DOI: 10.1210/clinem/dgab592
5.
5. Burg van den E. H., Stoop R. Neuropeptide signalling in the central nucleus of the amygdala // Cell and Tussie Research. 2019. Vol. 375. Issue 1. P. 93–101. DOI: 10.1007/s00441-018-2862-6
6.
6. Cheng Y. A human carboxypeptidase E/NF-α1 gene mutation in an Alzheimer’s disease patient leads to dementia and depression in mice / Y. Cheng, N. X. Cawley, T. Yanik et al. // Translational Psychiatry. 2016. Vol. 6. Art. e973. DOI: 10.3389/fnmol.2022.918852
7.
7. Cruz-Pereira J. S. Depression’s Unholy Trinity: Dysregulated Stress, Immunity, and the Microbiome / J. S. Cruz-Pereira, K. Rea, Y. M. Nolan et al. // Annual Review of Psychology. 2020. Vol. 71. P. 49–78. DOI: 10.1146/annurev-psych-122216-011613
8.
8. Han W. Angiotensin-Ⅱ and angiotensin-(1-7) imbalance affects comorbidity of depression and coronary heart disease / W. Han, Z. Wei, R. Dang et al. // Peptides. 2020. Vol. 131. Art. 170353. DOI: 10.1016/j.peptides.2020.170353
9.
9. Ji L. Dissecting carboxypeptidase E: properties, functions and pathophysiological roles in disease / L. Ji, H.-T. Wu, X.-Y. Qin et al. // Endocrine Connections. 2017. Vol. 6. Issue 4. P. 18–38. DOI: 10.1530/EC-17-0020
10.
10. Joëls M. Effects of chronic stress on structure and cell function in rat hippocampus and hypothalamus / M. Joëls, H. Karst, D. Alfarez et al. // Stress. 2004. Vol. 7. Issue 4. P. 221–231. DOI: 10.1080/10253890500070005
11.
11. Kalinina E., Varlamov O., Fricker L. D. Analysis of the carboxypeptidase D cytoplasmic domain: Implications in intracellular trafficking // Journal of Cellular Biochemistry. 2002. Vol. 85. Issue 1. P. 101–111. DOI: 10.1002/jcb.10112
12.
12. Malyshev A. V. Peptide LCGA-17 Attenuates Behavioral and Neurochemical Deficits in Rodent Models of PTSD and Depression / A. V. Malyshev, I. A. Sukhanova, V. M. Ushakova et al. // Pharmaceuticals. 2022. Vol. 15. Issue 4. Art. 462. DOI: 10.3390/ph15040462
13.
13. Mizushige T. Neuromodulatory peptides: Orally active anxiolytic-like and antidepressant-like peptides derived from dietary plant proteins // Peptides. 2021. Vol. 142. Art. 170569. DOI: 10.1016/j.peptides.2021.170569
14.
14. Rana T. Exploring the role of neuropeptides in depression and anxiety / T. Rana, T. Behl, A. Sehgal et al. // Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2022. Vol. 114. Art. 110478. DOI: 10.1016/j.pnpbp.2021.110478
15.
15. Song L., Fricker L. D. Purification and characterization of carboxypeptidase D, a novel carboxypeptidase E-like enzyme, from bovine pituitary // Journal of Biological Chemistry. 1995. Vol. 270. Issue 42. P. 25007–25013. DOI: 10.1074/jbc.270.42.25007
16.
16. Stornetta R. L. Neurochemistry of bulbospinal presympathetic neurons of the medulla oblongata // Journal of Chemical Neuroanatomy. 2009. Vol. 38. Issue 3. P. 222–230. DOI: 10.1016/j.jchemneu.2009.07.005
17.
17. Tartt A. N. Dysregulation of adult hippocampal neuroplasticity in major depression: pathogenesis and therapeutic implications / A. N. Tartt, M. B. Mariani, R. Hen et al. // Molecular Psychiatry. 2022. Vol. 27. P. 2689–2699. DOI: 10.1038/s41380-022-01520-y
18.
18. Xiao L., Loh Y. P. Neurotrophic Factor-α1/Carboxypeptidase E Functions in Neuroprotection and Alleviates Depression // Frontiers in Molecular Neuroscience. 2022. Vol. 15. Art. 918852. DOI: 10.3389/fnmol.2022.918852