Authors
- Stolyarova Angela Nikolaevna
- Yesaulenko Elena Evgenievna Doctor of Biological Sciences
Annotation
The relevance of the study is due to the fact that ischemia-reperfusion liver damage occurs in various clinical situations, such as shock of various aetiologies and other
circulatory disorders, liver resections, transplantations and other surgical interventions that require preventive shutdown of blood flow. A number of approaches have been proposed for the prevention of liver damage, which are referred to under the general term preconditioning. The purpose of this paper is to evaluate the possibility of preconditioning ischemiareperfusion syndrome using pro-oxidants. The study was carried out on 5 groups of white nonlinear rats, which underwent the experimental modelling of complete vascular isolation of the liver for 20 minutes with 3 hours of reperfusion without correction and with preliminary administration of solutions of tert-butyl hydroperoxide and/or ascorbic acid. As a result of the studies, it is revealed that the introduction of substances with pro- or antioxidant action can have a protective effect on the development of ischemia-reperfusion liver damage. This is manifested by lower activity values of enzyme markers of hepatocyte cytolysis in the blood plasma and higher values of indices of the functional state of the antioxidant protection system. During preconditioning with a 0,5 % solution of tert-butyl hydroperoxide, ALT activity was by 3,5 times lower than that of the comparison group, AST — by 2,1 times, LDH — by 4,7 times respectively. The possibility of pharmacological preconditioning by means of the multidirectional effects of the substances mentioned above was also confirmed by the leveling of the cytoprotective effect when combined with tert-butyl hydroperoxide after ascorbic acid. The data obtained indicate the prospect of searching not only for ways to use antioxidants in therapeutic practice, but also serve as justification for the possibility of pro-oxidant therapy.
How to link insert
Stolyarova, A. N. & Yesaulenko, E. E. (2023). INFLUENCE OF PRECONDITIONING WITH PRO-/ANTIOXIDANTS ON THE DEVELOPMENT OF ISCHEMIA-REPERFUSION LIVER DAMAGE IN EXPERIMENTAL CONDITIONS Bulletin of the Moscow City Pedagogical University. Series "Pedagogy and Psychology", 2023, №4 (52), 38. https://doi.org/10.25688/2076-9091.2023.52.4.03
References
1.
1. Karpishchenko A. I. Handbook. Medical Laboratory Technology. SPb.: Intermedika, 2002. 600 р. (In Russ.).
2.
2. Popov К. А. Prooxidant preconditioning of ischemic-reperfusion liver damage in experiment / К. А. Popov [et al.] // Medical News of North Caucasus. 2022. T. 17 (1). Р. 56–59. (In Russ.). DOI: 10.14300/mnnc.2022.17015
3.
3. Sementsov A. S. The role of reactive oxygen species in the infarct-limiting effect of hypoxic preconditioning / A. S. Sementsov [et al.] // Regional blood circulation and microcirculation. 2021. Т. 20 (2). P. 87–91. (In Russ.). DOI: 10.24884/1682-6655-2021-20- 2-87-91
4.
4. Choi E. K., Lim D. G. Hepatic ischemia-reperfusion injury with respect to oxidative stress and inflammatory response: a narrative review // J Yeungnam Med Sci. 2023. Vol. 40 (2). P. 115–122. DOI: 10.12701/jyms.2022.00017
5.
5. Hausenloy D. J., Yellon D. M. Ischaemic conditioning and reperfusion injury // Nat Rev Cardiol. 2016. Vol. 13 (4). P. 193–209. DOI: 10.1038/nrcardio.2016.5
6.
6. Lin J. Protective effects of ischemic preconditioning protocols on ischemia-reperfusion injury in rat liver / J. Lin [et al.] // J Invest Surg. 2020. Vol. 33 (9). P. 876–883. DOI: 10.1080/08941939.2018.1556753
7.
7. Nakazato P. C. G. Liver ischemia and reperfusion injury. Pathophysiology and new horizons in preconditioning and therapy / P. C. G. Nakazato [et al.] // Acta Cir Bras. 2018. Vol. 33 (8). P. 723–735. DOI: 10.1590/s0102-865020180080000008
8.
8. Oliveira G. C. Impacts of ischemic preconditioning in liver resection: systematic review with meta-analysis / G. C. Oliveira [et al.] // International Journal of Surgery. 2023. Vol. 109 (6). P. 1720–1727. DOI: 10.1097/JS9.0000000000000243
9.
9. Panconesi R. Mitochondria and ischemia reperfusion injury / R. Panconesi [et al.] // Curr Opin Organ Transplant. 2022. Vol. 27 (5). Р. 434–445. DOI: 10.1097/MOT.0000000000001015
10.
10. Qing Z. S. Protective effect of ischemia preconditioning on ischemia-reperfusion injury in rat liver transplantation / Z. S. Qing [et al.] // Genet Mol Res. 2015. Vol. 14 (2). P. 3018–3025. DOI: 10.4238/2015.April.10.12
11.
11. Solhjoo S. Oxidative stress in the mitochondrial matrix underlies ischemia/reperfusion-induced mitochondrial instability / S. Solhjoo [et al.] // J Biol Chem. 2023. Vol. 299 (1). P. 102780. DOI: https://doi.org/10.1016/j.jbc.2022.102780
12.
12. Spoelstra-de Man A. M. E., Elbers P. W. G., Oudemans-van Straaten H. M. Making sense of early high-dose intravenous vitamin C in ischemia/reperfusion injury // Crit Care. 2018. Vol. 22. P. 70. DOI: 10.1186/s13054-018-1996-y
13.
13. Wu L., Li Z., Yao Y. Hydrogen peroxide preconditioning is of dual role in cardiac ischemia/reperfusion // European Journal of Pharmacology. 2023. Vol. 947. Р. 175684. DOI: 10.1016/j.ejphar.2023.175684