Authors
- Tymoshenko Yana Evgenievna
- Yesaulenko Elena Evgenievna Doctor of Biological Sciences
- Shevchenko Alexey Stanislavovich
Annotation
Currently, there are no available pharmacological methods of metabolic correction that can provide reliable organ protection during ischemia-reperfusion damage. One
of the possible ways of metabolic correction of such damage is the regulation of the activity of oxidative decarboxylation of pyruvate. The purpose of the study is to determine the nature of the effect of sodium dichloroacetate on the development of ischemia-reperfusion damage of the liver under conditions of vascular isolation of the parenchyma in rats. The study was performed on seven groups of rats: a control group, comparison groups (with different models of liver ischemia-reperfusion) and experimental groups, which were administered sodium dichloroacetate 300 mg/kg intraperitoneally before ischemia. As a result of the studies, experimental data were obtained for the first time confirming the decrease in pyruvate dehydrogenase activity after liver ischemia-reperfusion by 70–72 %. This may be one of the key links in the pathogenesis of the developing pathological process, blocking the use of glucose in energy exchange after the blood flow restoration. The use of sodium dichloroacetate was accompanied by an increase in pyruvate dehydrogenase activity by 4,7–5,0 relative to the corresponding comparison groups at the reperfusion stage. Against the background of preliminary administration of sodium dichloroacetate, a decrease in the severity of the cytolytic syndrome was observed according to the determination
of the activity of aminotransferases and LDH in the blood plasma, which was 2,0–3,0 times lower than the corresponding indicators in rats in which ischemia-reperfusion
was modeled without correction. The data obtained confirm the possibility of reducing the level of hepatocyte cytolysis as well as the level of lactic acidosis and normalizing the prooxidant-antioxidant balance under the condition of preconditioning with sodium dichloroacetate of complete or partial vascular isolation of the rat liver. In this case, the pyruvate dehydrogenase complex can be considered a potential target for mitochondrial cytoprotectors, the impact of which can effectively increase cell resistance to hypoxia.
How to link insert
Tymoshenko, Y. E., Yesaulenko, E. E. & Shevchenko, A. S. (2023). INFLUENCE OF DICHLOROACETATE ON PYRUVATE DEHYDROGENASE ACTIVITY AND LIVER DAMAGE DURING ISCHEMIA-REPERFUSION IN EXPERIMENTAL CONDITIONS Bulletin of the Moscow City Pedagogical University. Series "Pedagogy and Psychology", 2023, №4 (52), 50. https://doi.org/10.25688/2076-9091.2023.52.4.04
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